229 Elaboration of reconstructed sheep epidermis
نویسندگان
چکیده
منابع مشابه
Reconstructed Human Epidermis: an Efficient Prediction Tool
RHE has the ultimate advantage to grow donor epidermal cells in a serumfree culture environment: after rapidly proliferating preparative keratinocyte cultures have been obtained, the yielded epidermal cells are seeded on inert filter substrates that are lifted to the air-liquid interface in a humidified-air incubator. A fully-defined nutrient medium feeds the basal cells through the filter subs...
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The intercellular lipid regions in the stratum corneum (SC), the outermost layer of the skin, form the major barrier for diffusion of substances through the skin. The barrier function of in vitro reconstructed epidermis is still impaired. With respect to further optimization of the model, it is necessary to characterize its stratum corneum lipid structure. In this study, small and wide angle X-...
متن کاملEvaluation of the Safety and Toxicological Profile of RheoSprayTM on Reconstructed Human Epidermis
The human epidermis is a common site for local and systemic delivery of medication. This study aims to compare the safety and toxicological profile of RheoSpray, a proprietary sprayable topical gel compounding base, to the profile of the positive control Triton X-100, using a 3D model of the human epidermis tissue. Results have demonstrated that RheoSpray presented an ET50 superior to 24 hr, as...
متن کاملModel for human skin reconstructed in vitro composed of associated dermis and epidermis.
CONTEXT AND OBJECTIVE The technique of obtaining human skin with dermis and epidermis reconstructed from cells isolated from patients can enable autologous skin grafting on patients with few donor sites. It also enables in vitro trials on chemicals and drugs. The objective of this work was to demonstrate a method for obtaining human skin composed of associated dermis and epidermis, reconstructe...
متن کاملTopically applied lactic acid increases spontaneous secretion of vascular endothelial growth factor by human reconstructed epidermis.
BACKGROUND Alpha-hydroxy acids (AHAs) are widely used for the treatment of hyperkeratotic skin disorders and photodamaged skin. OBJECTIVES To investigate the effect of lactic acid (LA) on the secretion of cytokines by keratinocytes (KCs) of human reconstructed epidermis. METHODS Creams containing 1.5%, 3% or 5% LA or vehicle controls were topically applied on to human epidermal equivalents ...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2019
ISSN: 0022-202X
DOI: 10.1016/j.jid.2019.07.230